Fig. 5
From: Tum1 is involved in the metabolism of sterol esters in Saccharomyces cerevisiae
Mouse TST protein cannot complement Tum1 deficiency in the tRNA thiolation pathway. The tRNA thiolation status was analysed in the wild-type strain (WT), the strain without Uba4 protein (uba4Δ), and in strains transformed with empty plasmids (WT[empty], tum1Δ[empty]) and with plasmids with inserted yeast and mouse homologues (tum1Δ[TUM1], tum1Δ[Tst]). Total tRNA was resolved by urea polyacrylamide-gel electrophoresis in the presence of APM