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Fig. 5 | BMC Microbiology

Fig. 5

From: Tum1 is involved in the metabolism of sterol esters in Saccharomyces cerevisiae

Fig. 5

Mouse TST protein cannot complement Tum1 deficiency in the tRNA thiolation pathway. The tRNA thiolation status was analysed in the wild-type strain (WT), the strain without Uba4 protein (uba4Δ), and in strains transformed with empty plasmids (WT[empty], tum1Δ[empty]) and with plasmids with inserted yeast and mouse homologues (tum1Δ[TUM1], tum1Δ[Tst]). Total tRNA was resolved by urea polyacrylamide-gel electrophoresis in the presence of APM

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