Table 2 Primer sequences used for qPCR
From: Acute Penicillium marneffei infection stimulates host M1/M2a macrophages polarization in BALB/C mice
Gene | Sequences | Product size (bp) |
|---|---|---|
iNOS | 5′-CAAGCACATTTGGGAATGGAGA-3′ 5′-CAGAACTGAGGGTACATGCTGGAG-3’ | 136 |
Arg1 | 5’-AGCTCTGGGAATCTGCATGG-3′ 5′-ATGTACACGATGTCTTTGGCAGATA-3’ | 125 |
GAPDH | 5’-TGTGTCCGTCGTGGATCTGA-3′ 5′-TTGCTGTTGAAGTCGCAGGAG-3’ | 150 |
T-bet | 5’-CATGGAGAACGGAGAATGGA-3′ 5′-TGGACAGGGGAAGAGAGCA-3’ | 118 |
GATA-3 | 5’-GGATGTAAGTCGAGGCCCAAG-3′ 5′-ATTGCAAAGGTAGTGCCCGGTA-3’ | 117 |
β-actin | 5’-CATCCGTAAAGACCTCTATGCCAAC-3′ 5′-ATGGAGCCACCGATCCACA-3’ | 171 |