Fig. 6

Effect of CYA on alveolar macrophage polarization in four weeks P. marneffei infected mice. Alveolar macrophages from four weeks P. marneffei infected (PM) mice were purified and treated as described above. a, ELISA. The supernatant from alveolar macrophages with different treatments were collected and analyzed for IL-12, TNF-α and IL-10 (M1 and M2a-related cytokines) levels. The data are represented as Mean ± SD (N = 4) and analyzed using ANOVA. *P < 0.05 and ^# P > 0.05. b, qRT-PCR. Alveolar macrophages from infected mice were treated with different agents and collected and subjected to qRT-PCR for iNOS and Arg1 gene (M1 and M2a-related key enzymes) expression. The data are expressed as mean ± SD (N = 5) and analyzed using ANOVA. *P < 0.05 and ^# P > 0.05. c, Griess and arginase activity assays. Alveolar macrophages and conditioned media were collected and subjected to Urea and NO production using arginase and Griess activity assays, respectively. The data are expressed as mean ± SD (N = 5) and analyzed using ANOVA. *P < 0.05 and ^# P > 0.05