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Fig. 5 | BMC Microbiology

Fig. 5

From: Acute Penicillium marneffei infection stimulates host M1/M2a macrophages polarization in BALB/C mice

Fig. 5

Effect of CYA on alveolar macrophage polarization in two weeks P. marneffei infected mice. Alveolar macrophages from two weeks P. marneffei infected (PM) mice were purified and treated as described in Methods. No stimuli served as a negative control (PM group), IFN-γ + LPS were included as the M1 positive control (PM- IFN-γ + LPS group), IL-4 was included as the M2a positive control (PM-IL-4 group) and CYA was included as an experimental group (PM-CYA group). a, ELISA. The supernatant from alveolar macrophages with different treatments were collected and analyzed for IL-12, TNF-α and IL-10 (M1 and M2a-related cytokines) levels. The data are represented as Mean ± SD (N = 4) and analyzed using ANOVA. *P < 0.05 and # P > 0.05. b, qRT-PCR. Alveolar macrophages after different treatments were collected and subjected to qRT-PCR for iNOS and Arg1 gene (M1 and M2a-related key enzymes) expression. The data are expressed as mean ± SD (N = 5) and analyzed using ANOVA. *P < 0.05. c, Griess and arginase activity assays. Alveolar macrophages and conditioned media were collected and subjected to Urea and NO production using arginase and Griess activity assays, respectively. The data are expressed as mean ± SD (N = 5) and analyzed using ANOVA. *P < 0.05 and # P > 0.05

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