Fig. 4
From: Acute Penicillium marneffei infection stimulates host M1/M2a macrophages polarization in BALB/C mice
M1 and M2a-related cytokines and key enzymes expression after P. marneffei infection. a, ELISA. Supernatant from alveolar macrophages of normal control (N) and P. marneffei infected (PM) mice were collected and analyzed for IL-12, TNF-α and IL-10 (M1 and M2a-related cytokines) levels. The data are represented as mean ± SD (N = 4) and analyzed using ANOVA. *P < 0.05 and # P > 0.05. b, qRT-PCR. Alveolar macrophages were collected from normal control (N) and P. marneffei infected (PM) mice and subjected to qRT-PCR for iNOS and Arg1 gene (M1 and M2a-related key enzymes) expression. The data are expressed as mean ± SD (N = 5) and analyzed using ANOVA. *P < 0.05. c, Griess and arginase activity assays. Supernatant from alveolar macrophages of normal control (N) and P. marneffei infected (PM) mice were subjected to NO production using Griess activity assays, and alveolar macrophages were subjected to Urea production using arginase activity assays, respectively. The data are expressed as mean ± SD (N = 5) and analyzed using ANOVA. *P < 0.05 and # P > 0.05