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Table 1 Bacterial strains and plasmids used in this study

From: RpoN2- and FliA-regulated fliTX is indispensible for flagellar motility and virulence in Xanthomonas oryzae pv. oryzae

Strain or plasmid Relevant characteristicsa Source or Reference
Escherichia coli
 DH5α supE44 ΔlacU169(Φ80lacZΔM15) hsdR17 recA1 endA1 gyrA96 thi-1 relA1 [49]
 BL21 For protein expression Novagen
Xanthomonas oryzae pv. oryzae
 PXO99A Wildtype strain, Philippine race 6 Lab collection
 ∆fliTX fliTX gene deletion mutant derived from PXO99A This study
 ∆fliTX-C Complementary strain of ∆fliTX, Apr This study
 ∆rpoN2 rpoN2 gene deletion mutant derived from PXO99A, Gmr Our lab
 ∆rpoN2-C Complementary strain of ∆rpoN2, Apr Our lab
 ∆fleQ fleQ gene deletion mutant derived from PXO99A, Gmr Our lab
 ∆fleQ-C Complementary strain of ∆fleQ, Apr Our lab
 ∆fliA fliA gene deletion mutant derived from PXO99A, Gmr Our lab
 ∆fliA-C Complementary strain of ∆fliA, Apr Our lab
Plasmid
 pMD18-T Cloning vector, Apr TaKaRa, Tokyo
 pKMS1 Suicidal vector carrying sacB gene for non-marker mutagenesis, Kmr [45]
 pBBR1MCS-4 Broad-host range expression vector, Apr [50]
 pHT304BZ Promoterless lacZ vector, Apr [41]
 pHTpS pHT304BZ derivative carrying the promoter region of fliS, Apr This study
 pHM1 Broad-host range expression vector, Spr [42]
 pH-fliSp-lacZ pHM1 derivative carrying the promoter region of fliS and promoterless lacZ, Spr This study
 pET-28a Expression vector to generate a N-terminal His6 tag, Kmr Haigene
 pET-fliTX pET-28a derivative carrying fliTX, Kmr This study
 pPROBE-AT broad-host-range vector carrying a promoter-less gfp gene, Apr [47]
 pPhrpG pPROBE-AT derivative carrying the promoter region of hrpG and promoterless gfp, Apr This study
 pPhrpX pPROBE-AT derivative carrying the promoter region of hrpX and promoterless gfp, Apr This study
 pPhpa1 pPROBE-AT derivative carrying the promoter region of hpa1 and promoterless gfp, Apr Our lab
  1. aApr, Kmr, Spr, and Gmr indicate resistant to ampicillin, kanamycin, spectinomycin and gentamicin, respectively