Fig. 6
Transcription of T3SS-related genes in Xanthomonas oryzae pv. oryzae strains. a The relative expression of T3SS-related genes was detected by qRT-PCR in PXO99A, ∆fliTX and ∆fliTX-C strains. Fold changes of each gene was calculated using the 2-∆∆Ct method. b Promoter activity of hrpG, hrpX and hpa1 in PXO99A, ∆fliTX and ∆fliTX-C strains. The promoter of hrpG, hrpX and hpa1 were ligated to pPROBE-AT, a broad-host-range vector carrying a promoter-less gfp gene, resulting in plasmids pPhrpG, pPhrpX and pPhpa1, respectively. These plasmids, pPhrpG, pPhrpX and pPhpa1, were transferred to fliTX deletion mutant, complementary strain and wildtype strains by electroporation. Green fluorescent protein mean fluorescence intensity was determined for gated populations of bacterial cells by flow cytometry. Error bars represent standard deviations from three biological repeats, and asterisk indicates P < 0.05 by Student’s t test