Fig. 5

Effects of signaling molecule inhibitors on PepO-induced IL-8 and IP-10 release in BEAS-2B. a, b Cells were pre-treated with AG490 (20 μM), BAY11–7082 (10 μM), LY294002 (10 μM), SB203580 (20 μM), SP600125 (10 μM), or U0126 (20 μM) for 1 h, followed by incubation with PepO (10 μg /ml) for another 24 h. The release of IL-8 and IP-10 were determined by ELISA. DMSO (0.1%) was served as the vehicle control. Results are expressed as the mean ± SD (n = 3). Student’s t-test was performed to calculate the statistical significance (*p < 0.05 or **p < 0.01 or ***p < 0.001)