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Fig. 3 | BMC Microbiology

Fig. 3

From: IL-8 and IP-10 expression from human bronchial epithelial cells BEAS-2B are promoted by Streptococcus pneumoniae endopeptidase O (PepO)

Fig. 3

Effects of PepO on the activation of intracellular signaling molecules MAPKs and Akt in BEAS-2B. a BEAS-2B Cells were incubated with rPepO (10 μg /ml) for indicated times (0–120 min), lysed with lysis buffer, and the activation of p38, ERK, JNK and Akt were examined by western blot analysis. Three independent experiments with essentially identical outcome were performed, and representative blots were shown. b Densitometry quantification of blots from three tests were shown in histograms above. Phospho-Akt or phospho-MAPKs expression was normalized to β-actin for each sample, and they were graphed as fold change compared with medium control. Student’s t-test was performed to calculate the statistical significance (**p < 0.01 or ***p < 0.001)

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