Table 5 Linearity, PCR Efficiency and LOD of the pan-Alphavirus RT-PCR assay
From: Improved detection of genus-specific Alphavirus using a generic TaqMan® assay
CHIKV | WEEV | EEEV | VEEV | BFV | |
|---|---|---|---|---|---|
R2 | 0.994 | 0.998 | 0.995 | 0.993 | 0.991 |
Slope | −3.39 | −3.81 | −3.77 | −3.55 | −3.67 |
Intercept | 39.49 | 44.58 | 46.92 | 43.35 | 41.10 |
Efficiency (%) | 97.24 | 82.99 | 84.18 | 91.35 | 87.12 |
LOD (copies/run) | 26.6 | 59.5 | 42.6 | 111.2 | 87.5 |