Table 3 List of primers and probe assayed in RT-PCR reactions
From: Improved detection of genus-specific Alphavirus using a generic TaqMan® assay
RT-PCR MiX | Forward primer at final concentration of: | Reverse primers at final concentration of: | Probe at final concentration of: |
|---|---|---|---|
#1 primers from Grywna (2010) | F1, 500 nM | R1, 500 nM | P1, 250 nM |
#2 primers with deoxy- INOSINE | F2A, 500 nM | R2A, 250 nM +R3A, 250 nM +R4A, 250 nM | P1, 250 nM |
#3 primers without deoxy-INOSINE | F2B, 500 nM | R2B, 250 nM +R3B, 250 nM +R4B, 250 nM | P1, 250 nM |
#4 primers from Grywna (2010) | F1, 500 nM | R1, 500 nM | P2, 250 nM |
#5 primers with deoxy- INOSINE | F2A, 500 nM | R2A, 250 nM +R3A, 250 nM +R4A, 250 nM | P2, 250 nM |
#6 primers without deoxy-INOSINE | F2B, 500 nM | R2B, 250 nM +R3B, 250 nM +R4B, 250 nM | P2, 250 nM |