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Fig. 4 | BMC Microbiology

Fig. 4

From: Development of a new fluorescent reporter:operator system: location of AraC regulated genes in Escherichia coli K-12

Fig. 4

Relative cellular location of AraC regulated promoters in the presence and absence of inducer. The distances between fluorescent foci and the nearest cell pole was measured in 300 individual cells containing a single fluorescent foci derived from FROS probes adjacent to (a) araBAD, strain LR06, (b) araJ, strain LR39 and (c) araFGH, strain LR38. Distances are plotted, relative to cell length, in the presence and absence of the inducer, arabinose. For these experiments, araBAD and araJ were tagged with a LacO array and araFGH was tagged with a MalO array. The experiment was repeated on 3 separate occasions, with the same outcome observed. Associated P-values for uninduced compared to induced cells are: for araBAD, 0.556; for araJ, 0.252; and for araF, 0.005

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