Fig. 7

Distribution of putative HisN and Cg0911 orthologs within Bacteria. Orthologs were identified by BlastP using the C. glutamicum ATCC 13032 HisN and Cg0911 protein sequences as query within NCBI’s non-redundant protein sequences database (nr). A BlastP score ≥ 125 and a sequence coverage ≥ 80% were set as cut-off for identification. All hits were additionally checked for the HolPase motif 5 identified in this study (Fig. 5). Presence of a HisN or Cg0911 ortholog in at least one species is indicated on genus level by a green background or an orange surrounding, respectively. Since the actinobacterial branch focuses on the distribution of Cg0911 orthologs, display of HisN orthologs is reduced to family level and above. The same colors were applied on the family level and above if HisN or Cg0911 orthologs were identified in at least three or half of the entities of the lower level. A red background indicates that no HisN ortholog was found with the applied cut-off or did not possess the HolPase motif. The expected presence of a PHP-type HolPase [20] is marked with a blue surrounding and was spot-checked using the B. subtilis HisJ protein sequence via BlastP. The expected presence of a bifunctional HAD-type HolPase [12] is marked with a purple surrounding and was spot-checked using the E. coli His(NB) protein sequence via BlastP. Other kingdoms were not included in the analysis