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Fig. 4 | BMC Microbiology

Fig. 4

From: Sequence-based identification of inositol monophosphatase-like histidinol-phosphate phosphatases (HisN) in Corynebacterium glutamicum, Actinobacteria, and beyond

Fig. 4

Enzyme characteristics of HisN (■) and Cg0911 (▲). a Effect of different pH values. The activity at pH 7.35 was set to 100% for each protein individually. Assay conditions: 30 °C, 5 mM MgSO4, 500 μM HolP, n = 4. b Effect of incubation temperature. The activity at 30 °C was set to 100% for each protein individually. Assay conditions: pH = 7.35, 5 mM MgSO4, 500 μM HolP, n = 4. c Influence of inorganic phosphate (Pi) and d influence of l-histidinol. The activity without the addition of Pi or l-histidinol was set to 100% for each enzyme individually. Assay conditions: 30 °C, pH = 7.35, 5 mM MgSO4, 250 μM (HisN) and 500 μM (Cg0911) HolP. 1 M TEA-puffer instead of 0.1 M was used in the l-histidinol experiments. n = 3

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BMC Microbiology

ISSN: 1471-2180

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