Fig. 5From: Hypothetical protein Cpn0423 triggers NOD2 activation and contributes to Chlamydia pneumoniae-mediated inflammationEffects of Cpn0423 on pulmonary inflammation in vivo. A, Histopathological analysis of lung tissues from mice of all the experimental groups were performed at days 15 after intranasal administration (haematoxylin and eosin staining, panels a-d magnification 100×; panels e-h: magnification 400×). Mice were treated with PBS (a & e), PTR (b & f), Cpn0423 (50 mg/kg) (c & g), and PTR + Cpn0423 (50 mg/kg) (d & h). B, Results of histological scoring for pulmonary inflammatory changes of lung sections from different groups of mice. A score of 0–3 was adjudged to the tissue sections of the mice as described in Materials and methods. C, The total BALF cells in each group. D, The percentage of macrophages (Mϕ) and polymorphonuclear leukocyte (PMN) among total BALF cells. E, Levels of the cytokines IL-6 and TNF-α in BALF were measured using enzyme-linked immunosorbent assay. Each error bar represented mean ± SD. Two-tailed unpaired student t test was used in Fig. 5. E-H, ** P < 0.01, *** P < 0.001. n = 8 mice in PBS Group, n = 7 mice in PTR-control Group, n = 8 mice in Cpn0423 group, n = 6 mice in Cpn0423 + PTR group. PTR: Protein Transfection Reagent. Red arrowhead indicates inflammatory cells infiltrationBack to article page