Fig. 3

Cpn0423 fusion protein induces cytokine production. Bone marrow-derived macrophages (BMDMs, A-C) were treated with Cpn0423 in the presence of PTR. Cells plated in 96-wells were treated for 18-24 h and the media were harvested for cytokines protein measurement. a Dose course of Cpn0423-induced MIP-2 production in BMDMs. b Dose course of Cpn0423-induced IL-6 production in BMDMs. c Effect of polymyxin B sulfate (PMB, a LPS neutralizer) on Cpn0423 MIP-2 production. Cpn0423 (10 μg/ml) or LPS (10 ng/ml) was incubated with 50 μg/ml PMB for 30 min at 4 °C before treatment. d Effect of PTR on Cpn0423-induced MIP-2 production. BMDMs were treated with 10 μg/ml Cpn0423 pre-treated with or without PTR (None group). Each error bar represented Mean ± SD. Two-tailed unpaired student t test was used in Fig. 3.A-D, * P < 0.05, ** P < 0.01, *** P < 0.001. n = 3 in each group. PMB: polymyxin B sulfate; PTR: Protein Transfection Reagent