Fig. 2

Immunofluorescence detection of Cpn0423 in the C. pneumoniae-infected Hep-2 cells. Hep-2 cells infected with C. pneumoniae (A, panels a-d) or uninfected Hep-2 cells (A, panels e-h) were stained with mouse anti-Cpn0423 fusion protein antibodies visualized with a goat anti-mouse IgG-conjugated Cy3 (red). Cells were co-stained with a rabbit anti-Cpn AR39 visualized with a Cy2-conjugated goat anti-rabbit IgG (green) and a DNA Hoechst dye (blue). B, Cpn0423 pAb was pre-absorbed with Cpn0423and CPAF fusion proteins respectively, followed by immunostaining as described for panel A. The antibody pre-absorption was carried out as previously described [25]. Note that anti-Cpn0423 antibody labeling were removed by pre-absorption with the corresponding (B, panel b) but not heterologous CPAF (B, panel c) fusion proteins