Fig. 1

Cloning, expression and purification of Cpn0423 of C. pneumoniae. a PCR products of Cpn0423 amplified from the C. pneumoniae strain AR-39. Lane M, DNA Marker; lanes 1 and 2, PCR amplified products of Cpn0423; lane 3, negative control. b Identification and restriction end nuclease analysis of the vector pGEX6p-2/Cpn0423. Lane M, DNA Marker; lane 1, pGEX6p-2 vector; lane 2, pGEX6p-2/Cpn0423 vector; lane 3, pGEX6p-2/Cpn0423 digested with Bam H I and Not I; lane 4, PCR amplified products of Cpn0423. c SDS-PAGE analysis of recombinant protein expressed in E. coli BL21 (DE3) cells. Lane M, protein marker; lane 1, supernatant fraction from cell lysate of bacteria transformed with pGEX6p-2/Cpn0423 with IPTG (1 mM) induction for 4 h; lane 2, PBS Washings; lane 3: Protein Cpn0423 first eluent from glutathione sepharose; lane 4: Purified protein Cpn0423 from glutathione sepharose. d Immunoblotting of Cpn0423: eluent buffer (Con.) and purified Cpn0423 were immunoblotted for Cpn0423