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Table 1 Microbial Culture and QPCR Analysis of Jejunal Lumen

From: The human jejunum has an endogenous microbiota that differs from those in the oral cavity and colon

Patient Bact. DNA Colonies Viability Aerobic Culture CFU Anaerobic Culture CFU
BGE/ml CFU/ml CFU/BGE Trypt-Blood Levine-EMB CDC-Blood PEA-Blood KV-Blood
% % % % %
A 3.1 × 107 1.6 × 105 0.005 7.9 0.0 52.6 39.5 0.0
B 2.9 × 107 4.7 × 105 0.016 6.8 69.1 3.20 14.3 6.6
C 2.4 × 107 4.2 × 105 0.018 18.1 27.2 34.4 20.0 0.3
D 1.3 × 107 7.9 × 105 0.061 49.7 9.9 34.8 5.0 0.6
E 1.3 × 107 3.1 × 105 0.024 26.3 0.8 41.3 30.1 1.5
F 8.5 × 106 2.2 × 104 0.003 2.7 0.0 43.3 54.0 0.0
G 7.9 × 106 1.1 × 105 0.014 29.7 0.0 48.8 21.6 0.0
H 5.5 × 106 5.2 × 106 0.945 9.7 1.0 71.1 18.1 0.1
I 5.2 × 106 3.1 × 104 0.006 21.3 0.0 65.6 13.1 0.0
J 4.7 × 106 1.5 × 106 0.319 5.1 0.1 43.6 51.2 0.0
K 4.5 × 106 3.0 × 106 0.667 27.1 25.1 36.8 11.0 0.0
L 3.2 × 106 7.9 × 106 2.469 34.5 0.6 23.7 39.8 1.4
M 3.2 × 106 2.2 × 104 0.007 6.2 0.0 29.2 64.6 0.0
N 2.3 × 106 2.0 × 105 0.087 5.7 0.0 85.7 8.6 0.0
O 2.1 × 106 8.4 × 105 0.400 16.2 0.0 68.1 12.7 3.0
P 1.3 × 106 2.8 × 104 0.022 11.1 0.0 74.1 14.8 0.0
Q 1.0 × 106 6.6 × 104 0.066 6.4 5.9 14.0 73.7 0.0
R 7.3 × 105 5.7 × 103 0.008 21.4 0.0 71.4 7.1 0.1
S 2.8 × 105 9.9 × 103 0.035 14.2 0.0 62.7 18.9 4.2
T 1.5 × 105 4.2 × 104 0.280 7.6 0.1 75.9 15.1 1.3
  1. Bacterial yields have been normalized to bacteria per ml of standard undiluted jejunal contents. Results were calibrated with a standard dilution series of purified E. coli DNA. Column 1 is the patient designation, ranked according to total live bacteria. Column 2 is the bacterial genome number estimated by quantitative PCR analysis of extracted DNA using pan-bacterial PCR primers for 16S rRNA genes. Column 3 represents live bacterial colonies, consisting of the sum of CFU/ml for all 5 culture media. Column 4 represents the viability fraction of a given sample. This is the ratio of CFU/BGE. Sample L is likely greater than 1 because of errors in the two measurements, or assumptions regarding average 16S gene number of the bacteria. Columns 5 through 9 represent the percent of total colony forming units (CFUs) counted on different culture media