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Table 1 Microbial Culture and QPCR Analysis of Jejunal Lumen

From: The human jejunum has an endogenous microbiota that differs from those in the oral cavity and colon

Patient

Bact. DNA

Colonies

Viability

Aerobic Culture CFU

Anaerobic Culture CFU

BGE/ml

CFU/ml

CFU/BGE

Trypt-Blood

Levine-EMB

CDC-Blood

PEA-Blood

KV-Blood

%

%

%

%

%

A

3.1 × 107

1.6 × 105

0.005

7.9

0.0

52.6

39.5

0.0

B

2.9 × 107

4.7 × 105

0.016

6.8

69.1

3.20

14.3

6.6

C

2.4 × 107

4.2 × 105

0.018

18.1

27.2

34.4

20.0

0.3

D

1.3 × 107

7.9 × 105

0.061

49.7

9.9

34.8

5.0

0.6

E

1.3 × 107

3.1 × 105

0.024

26.3

0.8

41.3

30.1

1.5

F

8.5 × 106

2.2 × 104

0.003

2.7

0.0

43.3

54.0

0.0

G

7.9 × 106

1.1 × 105

0.014

29.7

0.0

48.8

21.6

0.0

H

5.5 × 106

5.2 × 106

0.945

9.7

1.0

71.1

18.1

0.1

I

5.2 × 106

3.1 × 104

0.006

21.3

0.0

65.6

13.1

0.0

J

4.7 × 106

1.5 × 106

0.319

5.1

0.1

43.6

51.2

0.0

K

4.5 × 106

3.0 × 106

0.667

27.1

25.1

36.8

11.0

0.0

L

3.2 × 106

7.9 × 106

2.469

34.5

0.6

23.7

39.8

1.4

M

3.2 × 106

2.2 × 104

0.007

6.2

0.0

29.2

64.6

0.0

N

2.3 × 106

2.0 × 105

0.087

5.7

0.0

85.7

8.6

0.0

O

2.1 × 106

8.4 × 105

0.400

16.2

0.0

68.1

12.7

3.0

P

1.3 × 106

2.8 × 104

0.022

11.1

0.0

74.1

14.8

0.0

Q

1.0 × 106

6.6 × 104

0.066

6.4

5.9

14.0

73.7

0.0

R

7.3 × 105

5.7 × 103

0.008

21.4

0.0

71.4

7.1

0.1

S

2.8 × 105

9.9 × 103

0.035

14.2

0.0

62.7

18.9

4.2

T

1.5 × 105

4.2 × 104

0.280

7.6

0.1

75.9

15.1

1.3

  1. Bacterial yields have been normalized to bacteria per ml of standard undiluted jejunal contents. Results were calibrated with a standard dilution series of purified E. coli DNA. Column 1 is the patient designation, ranked according to total live bacteria. Column 2 is the bacterial genome number estimated by quantitative PCR analysis of extracted DNA using pan-bacterial PCR primers for 16S rRNA genes. Column 3 represents live bacterial colonies, consisting of the sum of CFU/ml for all 5 culture media. Column 4 represents the viability fraction of a given sample. This is the ratio of CFU/BGE. Sample L is likely greater than 1 because of errors in the two measurements, or assumptions regarding average 16S gene number of the bacteria. Columns 5 through 9 represent the percent of total colony forming units (CFUs) counted on different culture media