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Table 1 Bacterial strains and plasmids used in this study

From: Contribution of pilus type 2b to invasive disease caused by a Streptococcus agalactiae ST-17 strain

Strain or plasmid Relevant characteristic(s) Source or reference
E. coli
 DH5α F- φ80lacZΔM15 Δ(lacZYA-argF) U169 recA1 endA1 hsdR17 (rk-, mk+) phoA supE44 λ- thi-1 gyrA96 relA1 Invitrogen
 Mach1™-T1R F- φ80(lacZ)ΔM15 ΔlacX74 hsdR(rK-mK+) ΔrecA1398 endA1 tonA Invitrogen
S. agalactiae
 COH1 GBS wild type Rosini et al.
Δbp-1 COH1 knockout (KO) deleted of the gene coding for the backbone protein of pilus 1 (BP-1 or GBS80) Rosini et al.
Δbp-2b COH1 knockout (KO) deleted of the gene coding for the backbone protein of pilus 2b (BP-2b) This study
Δbp-2b+ Δbp-2b complemented with pAM401_ bp-2b This study
Plasmids
 pJRS233 6.0 kb; ColE1 ori; temperature-sensitive E. coli-streptococcal shuttle vector Perez-Casal et al.
 pJRS233_ bp-2b pJRS233-derived containing overlapping flanking sequences of bp-2b gene This study
 pAM401/gbs80P + T 11.5 kb; Cmr; ColE1 ori; E. Coli-streptococcal shuttle vector pAM401 containing promoter and terminator of gbs80 gene Rosini et al.
 pAM401_ bp-2b pAM401/gbs80P + T-derived containing entire bp-2b coding sequence This study