XC_0531 encodes a c-type cytochrome biogenesis protein and is required for pathogenesis in Xanthomonas campestris pv. campestris

Table 1 Strains and plasmids used in this study

Strains and Plasmids	Genotype/Properties	Reference/Resource
Escherichia coli
JM109	RecA1, endA1, gyrA96, thi, supE44, relA1△ (lac-proAB)/F′ [traD36, lacI ^q, lacZ△M15]	Our lab’s collection
ED8767	RecA, met,containing pRK2073,Spc^r	Our lab’s collection
M15	Kmlac ara gal mtl recA1 uvr1 [pREP4 lacI Kanr]	Our lab’s collection
Xanthomonos campestris pv. compestris
8004	Wild type; Rif ^r	Our lab’s collection
015F07	As 8004,but XC_3579::Tn5gusA5;Rif^r, Km^r	This work
045F12	As 8004, but XC_3579::Tn5gusA5;Rif^r, Km^r	This work
142E11	As 8004, but XC_3579::Tn5gusA5;Rif^r, Km^r	This work
0531pk	As 8004, but XC_0531::pK18mob; Rif^r, Km^r	This work
C0531	0531pk harboring pLA0531; Rif^r, Km^r, Tet^r	This work
Plasmids
pK18mob	pUC18 derivative, lacZα, Kan^r, mob site, suicide plasmid in X. campestris pv. campestris.	Our lab’s collection
pRK2073	Helper plasmid,Tra⁺, Mob⁺, ColE1, Spc^r.	Our lab’s collection
pET30a⁺	T7 promoter, Km^r	Our lab’s collection
pLC0531	pLAFR3 containing the whole XC_0531 gene;Tc^r	This work
pLAFR3	Broad host range cloning vector, Tc^r	Our lab’s collection

ISSN: 1471-2180