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Table 1 Strains and plasmids used in this study

From: XC_0531 encodes a c-type cytochrome biogenesis protein and is required for pathogenesis in Xanthomonas campestris pv. campestris

Strains and Plasmids Genotype/Properties Reference/Resource
Escherichia coli  
 JM109 RecA1, endA1, gyrA96, thi, supE44, relA1 (lac-proAB)/F′ [traD36, lacI q, lacZM15] Our lab’s collection
 ED8767 RecA, met,containing pRK2073,Spcr Our lab’s collection
 M15 Kmlac ara gal mtl recA1 uvr1 [pREP4 lacI Kanr] Our lab’s collection
Xanthomonos campestris pv. compestris  
 8004 Wild type; Rif r Our lab’s collection
 015F07 As 8004,but XC_3579::Tn5gusA5;Rifr, Kmr This work
 045F12 As 8004, but XC_3579::Tn5gusA5;Rifr, Kmr This work
 142E11 As 8004, but XC_3579::Tn5gusA5;Rifr, Kmr This work
 0531pk As 8004, but XC_0531::pK18mob; Rifr, Kmr This work
 C0531 0531pk harboring pLA0531; Rifr, Kmr, Tetr This work
Plasmids  
 pK18mob pUC18 derivative, lacZα, Kanr, mob site, suicide plasmid in X. campestris pv. campestris. Our lab’s collection
 pRK2073 Helper plasmid,Tra+, Mob+, ColE1, Spcr. Our lab’s collection
 pET30a+ T7 promoter, Kmr Our lab’s collection
 pLC0531 pLAFR3 containing the whole XC_0531 gene;Tcr This work
 pLAFR3 Broad host range cloning vector, Tcr Our lab’s collection