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Fig. 5 | BMC Microbiology

Fig. 5

From: XC_0531 encodes a c-type cytochrome biogenesis protein and is required for pathogenesis in Xanthomonas campestris pv. campestris

Fig. 5

Analysis of the periplasmic disulfide oxidoreductase activity and copper tolerance of Xanthomonas campestris pv. campestris strains. a Accumulation of proteins with reduced cysteines determined by the Ellman’s reaction analysis. Value is the mean ± standard deviation from three repeats; OD412 = optical density at 412 nm. b Dithiothreitol (DTT) sensitivity detection. Overnight culture (2 μl) of each X. campestris pv. campestris strain was spotted onto NYGA supplemented with DTT to a final concentration of 4 mM and incubated at 28 °C. Photographs were taken 2 days after inoculation. Three plates were inoculated in each experiment and each experiment was repeated three times. Data presented were from representative plates and similar results were obtained in all plates of an experiment or in the plates of other independent experiments. c Copper tolerance of wild-type Xcc 8004, 0531pk, C0531 and 015F07. Overnight cultures were cultured in NYG containing various concentrations of CuSO4 for 24 to 48 h at 30 °C. OD600 were test. The values are averages of three independent experiments. Different letters indicate significant differences at a level of α < 0.05 based on Duncan’s test by one way-ANOVA

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