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Table 3 The survival rates (%) of Caco-2 cells treated with various experimental groups using MTT and flow cytometry assays

From: The effects of probiotic Bacillus subtilis on the cytotoxicity of Clostridium perfringens type a in Caco-2 cell culture

Experimental groups

Cells survival (%)

MTT assay

Flow cytometry assay

Negative control

 

97.79 ± 2.3a

2

95.16 ± 3.59aA

95.32 ± 2.77bA

3

86.56 ± 3.69bA

92.71 ± 2.77cB

4

56.02 ± 1.8dA

61.42 ± 1.21eB

5

51.59 ± 1.71eA

57.65 ± 0.83fB

6

37.66 ± 2fA

42.29 ± 0.85gB

7

7.05 ± 0.41cA

13.08 ± 0.71dB

8

12.74 ± 0.73gA

18.74 ± 2.07hB

9

10.94 ± 0.57gA

16.32 ± 1.1jB

10

65.55 ± 1.75iA

71.66 ± 1.69kB

11

78 ± 2.67kA

85.42 ± 0.87mB

12

59.85 ± 2.58lA

67.45 ± 2.05nB

13

72.42 ± 2.39jA

78.33 ± 1.89lB

14

44.83 ± 1.67mA

50.05 ± 1.74oB

15

51.58 ± 1.67e

59.40 ± 1.37q

  1. Values are mean ± SD of five and three replications for MTT and flow cytometry assays, respectively. Different small and capital letters are representing the statistical difference between each column and rows, respectively (P < 0.05). Treatments are described in Table 1.