Skip to main content

Table 3 The survival rates (%) of Caco-2 cells treated with various experimental groups using MTT and flow cytometry assays

From: The effects of probiotic Bacillus subtilis on the cytotoxicity of Clostridium perfringens type a in Caco-2 cell culture

Experimental groups Cells survival (%)
MTT assay Flow cytometry assay
Negative control   97.79 ± 2.3a
2 95.16 ± 3.59aA 95.32 ± 2.77bA
3 86.56 ± 3.69bA 92.71 ± 2.77cB
4 56.02 ± 1.8dA 61.42 ± 1.21eB
5 51.59 ± 1.71eA 57.65 ± 0.83fB
6 37.66 ± 2fA 42.29 ± 0.85gB
7 7.05 ± 0.41cA 13.08 ± 0.71dB
8 12.74 ± 0.73gA 18.74 ± 2.07hB
9 10.94 ± 0.57gA 16.32 ± 1.1jB
10 65.55 ± 1.75iA 71.66 ± 1.69kB
11 78 ± 2.67kA 85.42 ± 0.87mB
12 59.85 ± 2.58lA 67.45 ± 2.05nB
13 72.42 ± 2.39jA 78.33 ± 1.89lB
14 44.83 ± 1.67mA 50.05 ± 1.74oB
15 51.58 ± 1.67e 59.40 ± 1.37q
  1. Values are mean ± SD of five and three replications for MTT and flow cytometry assays, respectively. Different small and capital letters are representing the statistical difference between each column and rows, respectively (P < 0.05). Treatments are described in Table 1.