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Table 1 Quality of results

From: Towards diagnostic metagenomics of Campylobacter in fecal samples

  Human fecal samples Chicken fecal samples Clinical fecal samples
qPCR r2 0.993 0.939 0.996
efficiency 78.3% 79.8% 104%
Whole genome sequencing of spiking organisms Genomic coverage of assembly 40.2× 113.8× N/A
N50 221,548 177,674 N/A
Number of contigs 281 67 N/A
MiSeq sequencing Cluster density (clusters/mm2) 991 1198 1326
Data quality after trimming Number of reads (billions) 2.96–5.57 4.27–8.47 2.74–6.20
Minimum read length 50 50 50
Maximum read length 251 251 251
Average read length 217–229 193–232 172–206
Data set size after down sampling Number of reads (billions) 2.96 4.27 2.74