Bp 576mn is unable to replicate in the human cell line HEK293 intracellular replication model and is not significantly cytotoxic. a Aminoglycoside protection assay revealed that Bp 576mn (orange square) was unable to replicate inside HEK293 cells compared to 576a WT (blue square). Strain 1026b (green triangle) and Bp82 (maroon triangle) were included as controls. Strain 576mn COMP was only partially returned to wild-type levels of replication (black diamonds). The symbols are the average of experimental data acquired in biological triplicate. The SEM is not visible at the scale presented. The differences between all strains at 2 h were not significant. Significance determined by Two-way ANOVA. ns = not significant, ** = p < 0.01 **** = p < 0.0001.
b Cytotoxicity of HEK293 cells after 48 h infection with the indicated strains. Significance determined by one-way ANOVA. ns = not significant, ** = p < 0.01 **** = p < 0.0001