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Fig. 1 | BMC Microbiology

Fig. 1

From: An avirulent Burkholderia pseudomallei ∆purM strain with atypical type B LPS: expansion of the toolkit for biosafe studies of melioidosis

Fig. 1

Strain background differences between Bp 1026b and 576a. a Silver stain of highly pure LPS from 1026b and 576a strain backgrounds and heat-killed lysate. b Coomassie stain of heat-killed cell lysate. c and d western blots using LPS type A specific mAb 4C7 and LPS type B specific mAb 5B4. e Western blot using day 28 serum from a rhesus macaque after aerosol challenge by Bp 1026b. Lanes: 1, FPLC-purified Bp82 Δwcb LPS; 2, FPLC-purified 576a Δwcb LPS; 1 lys, heat-killed lysate of Bp82; 2 lys, heat-killed lysate of 576mn. Chevrons indicate 80 kDa and 25 kDa as indicated

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BMC Microbiology

ISSN: 1471-2180

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