Identification of Enterococcus faecalis enzymes with azoreductases and/or nitroreductase activity

BMC Microbiology

Table 4 Summary of purified proteins activities

	Enzymes families	Reductase activity	FMN-dependence	NADPH	NADH
AzoA	Type 1: FMN-dependent NADH azoreductase	Azo +	+	+	++
AzoA	Type 1: FMN-dependent NADH azoreductase	Nitro +	+	++	+
EF0404	Group B: FMN-dependent NAD(P)H nitroreductase	Azo +	+	+	++
EF0404	Group B: FMN-dependent NAD(P)H nitroreductase	Nitro +	+	++	+
EF0648	Group B: FMN-dependent NAD(P)H nitroreductase	Azo -	NA	NA	NA
EF0648	Group B: FMN-dependent NAD(P)H nitroreductase	Nitro +	+	+	+
EF0655	FMN-dependent Nitroreductase	Azo -	NA	NA	NA
EF0655	FMN-dependent Nitroreductase	Nitro +	+	+	+
EF1181	Group A: FMN-dependent NADPH nitroreductase	Azo -	NA	NA	NA
EF1181	Group A: FMN-dependent NADPH nitroreductase	Nitro +	+	++	−

For each enzyme, it is reported whether it presents nitroreductase and/or azoreductase activity: +: Activity has been established by reduction of tested compound; −: No activity was observed; NA: not applicable
When activity was established, FMN-dependence and cofactor dependence/preference are indicated

ISSN: 1471-2180