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Table 3 Primers used for amplification and cloning of ef0404, ef0648, ef0655 and ef1181 in pQE30 plasmid

From: Identification of Enterococcus faecalis enzymes with azoreductases and/or nitroreductase activity

Targeted gene Primers Tm (°C) PCR amplicon
azoA cgggatccTCAAAATTATTAGTTGTTAAAGCACATCC 59.5 644 pb
acgcgtcgacATTTAGAATGTTTTACCGTATTCAGTTGC 60.4
ef0404 cgggatccACAACATATACAACGAATGATTTTTCAG 59.0 660 pb
acgcgtcgacTTTTATTGCCTATTCAAATGTCGTG 59.5
ef0648 cgggatccATGTATCAAGATGTTGTTCGCAGC 60.6 701 pb
acgcgtcgacCAATCACTTTGGATGTTTGTTCC 58.6
ef0655 cgggatccTCAAAATTTACTGAAATGATGAAAAACC 60.1 652 pb
acgcgtcgacGCTTTCACTCCTTTCCTCTTCG 59.7
ef1181 cgggatccAATCAAACAATCGAACAATTACTAAGTC 57.7 763 pb
acgcgtcgacCACGCTCTTTTGTTTAGACATC 58.2
  1. For each gene, primer couples are reported. Nucleotides identical to the gene sequence are in capital letters and nucleotide motifs required for cloning containing restriction sites BamHI or SalI are in lowercase