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Table 3 Primers used for amplification and cloning of ef0404, ef0648, ef0655 and ef1181 in pQE30 plasmid

From: Identification of Enterococcus faecalis enzymes with azoreductases and/or nitroreductase activity

Targeted gene

Primers

Tm (°C)

PCR amplicon

azoA

cgggatccTCAAAATTATTAGTTGTTAAAGCACATCC

59.5

644 pb

acgcgtcgacATTTAGAATGTTTTACCGTATTCAGTTGC

60.4

ef0404

cgggatccACAACATATACAACGAATGATTTTTCAG

59.0

660 pb

acgcgtcgacTTTTATTGCCTATTCAAATGTCGTG

59.5

ef0648

cgggatccATGTATCAAGATGTTGTTCGCAGC

60.6

701 pb

acgcgtcgacCAATCACTTTGGATGTTTGTTCC

58.6

ef0655

cgggatccTCAAAATTTACTGAAATGATGAAAAACC

60.1

652 pb

acgcgtcgacGCTTTCACTCCTTTCCTCTTCG

59.7

ef1181

cgggatccAATCAAACAATCGAACAATTACTAAGTC

57.7

763 pb

acgcgtcgacCACGCTCTTTTGTTTAGACATC

58.2

  1. For each gene, primer couples are reported. Nucleotides identical to the gene sequence are in capital letters and nucleotide motifs required for cloning containing restriction sites BamHI or SalI are in lowercase