Fig. 4
From: Identification of Enterococcus faecalis enzymes with azoreductases and/or nitroreductase activity
Nitroreductase activity of AzoA, EF0404, EF0648, EF0655, EF1181 and their cofactor preferences. a-b The proteins used were purified without their prosthetic group, that is purified using the denaturing/renaturing protocol. Nitroreductase activity is evaluated by following fluorescence intensity at 365/440 nm during 900 min in presence of 100 μmol.l−1 7NCCA and 10 mg.l−1 of indicated enzyme. To determine each enzyme preference toward cofactors, 7NCCA reduction is followed in presence of 500 μmol.l−1 NADPH (• • •) or NADH (− −). FMN is added at 5 μmol.l−1. The control wells contained the reaction buffer (7NCCA, FMN, NAD (P) H) without enzyme