Fig. 2
From: Identification of Enterococcus faecalis enzymes with azoreductases and/or nitroreductase activity
Phylogeny of azoreductase and nitroreductase proteins. Phylogenic tree was generated using a multiple alignment of proteins (Muscle alignment) which have experimentally shown azoreductase and/or nitroreductase activity. Nitroreductases of E. faecalis, studied here, are indicated in bold. A specific colour is attributed to each reductases family. For azoreductases, families type 1 to 3 are indicated. In dark blue, type 1 gathers FMN-dependent NADH azoreductases. In orange, type 2 regroups FMN-dependent NADPH azoreductases. In turquoise, type 3 are FMN-independent azoreductases. For nitroreductases, in red, group A represents oxygen-insensitive NADPH dependent nitroreductase. In green, group B gathers oxygen-insensitive NAD(P)H nitroreductase. The nomenclature is the following: first the protein name and then the species. The references of the protein sequences aligned are the following: Azo1_S. aureus:AAT29034.1; AzoA_E. faecalis:AAR38851.1; AzoA_P. kullae:AAO39146.1; AzoB_X. azovorans:AAM92125.2; AzoB_P. kullae:ADD80733.1; AzoR_R. sphaeroides:ABA81336.1.; AzoR_E. coli:AAC74494.1.; AzoR_G. stearothermophilus:AAD24436.1.; AzoR1_P. putida:AAN68474.1.; AzoR1_P. aeruginosa:AAG04174.1; AzoR1_B. subtilis:CAB13815.1.; AzoR2_P. aeruginosa:AAG05350.1; AzoR2_B. subtilis:CAB15359.1.; AzoR3_P. aeruginosa:AAG06611.1.; AzR_B. subtilis:CAB12762.1.; EF0404_E. faecalis:AAO80264.1.; EF0648_E. faecalis:AAO80473.1.; EF0655_E. faecalis:AAO80478.1; EF1181_E. faecalis:AAO80980.1; Frase1_V. fisheri:BAA04595.2.; FRP_V. harveyi:AAA21331.1.; NfrA_S. aureus:ABD29532.1.; NfrA1_B. subtilis:CAB15837.1.; NfsA_K. pneumoniae:ABR76318.1.; NfsA_E. coli:AAC73938.1.; NfsB_P. aeruginosa:AAG08575.1.; NfsB_E. coli:AAC73679.1.; NfsB_K. pneumoniae:ABR76003.1.; NtrA_S. aureus:ABD29959.1.; PnbA_L. plantarum:CCC77618.1.; PnrA_P. putida:AAM95986.1.; PnrB_P. putida:AAM95987.1.; SnrA_S. typhimurium:AAD18027.1.; YcnD_B. subtilis:BAA09018.1.; YdgI_B. cereus:AAP09971.1.; YdgI_B. subtilis:BAA19399.1.; YfkO_B. cereus:AAP08598.1.; YfkO_B. licheniformis:EWH23016.1.; YtjD_L. lactis:AAK06011.1.; NQO1_H. sapiens:AAB60701.1