TY - JOUR AU - García, Katherine AU - Yáñez, Cristian AU - Plaza, Nicolás AU - Peña, Francisca AU - Sepúlveda, Pedro AU - Pérez-Reytor, Diliana AU - Espejo, Romilio T. PY - 2017 DA - 2017/05/19 TI - Gene expression of Vibrio parahaemolyticus growing in laboratory isolation conditions compared to those common in its natural ocean environment JO - BMC Microbiology SP - 118 VL - 17 IS - 1 AB - Vibrio parahaemolyticus is an autochthonous marine bacterial species comprising strains able to grow in broth containing bile salts at 37 °C, a condition seldom found in the ocean. However, this condition is used for isolation in the laboratory because it is considered a necessary property for pathogenesis. In this context, revealing how gene expression enables V. parahaemolyticus to adapt to this particular condition –common to almost all V. parahaemolyticus isolates- will improve our understanding of the biology of this important pathogen. To determine the genes of V. parahaemolyticus differentially expressed when growing in isolation condition (37 °C, 0.9% NaCl, and 0.04% bile salts) referred to those at the temperature and salt concentration prevailing in ocean south of Chile (marine-like condition; 12 °C, 3% NaCl, and absence of bile salts) we used high-throughput sequencing of RNA. SN - 1471-2180 UR - https://doi.org/10.1186/s12866-017-1030-6 DO - 10.1186/s12866-017-1030-6 ID - García2017 ER -