Fig. 1
From: Characterization of novel Omp31 antigenic epitopes of Brucella melitensis by monoclonal antibodies
Reactivity of mAbs to Omp31 antigens of B. melitensis. (a) Binding of mAbs to 16mer overlapping peptides derived from Omp31 in Peptide-ELISA. (b) Identification of mAbs reacting with rOmp31 and NMP (native membrane protein extracts of B. melitensis M5–90) by Western-blot. (C) Reactivity of mAbs to NMP and SSP (supernatant of sonicated proteins of B. melitensis M5–90) by ELISA. NS3, an HCV NS3 peptide, recombinant protein and an un-related mAb to HCV NS3 were used as negative-controls, respectively. The dotted line indicates the level of cut off defined as mean + 2SD of OD value to negative controls