Table 4 Gene copy number of ETEC fimbriae genes, total ETEC fimbriae, UspA and toxins genes of diarrhea observed and health piglets detected by qPCR and HRM-qPCR methods. Data are presented as means of the log₁₀ number of gene copies/g wet feces ± SEM in positive samples ^A

K99 fimbriae (fan A)*

5.85 ± 0.14

(14/14)^D

7.52 ± 0.13

(14/14)

6.35 ± 0.04

(8/8)

3.99 ± 0.28

(6/8)

6.37 ± 0.05

(8/8)

3.97 ± 0.10

(5/8)

F41 fimbriae Subunit A*

6.67 ± 0.07

(14/14)

7.87 ± 0.13

(14/14)

4.46 + 0.07

(8/8)

3.62 ± 0.16

(6/8)

4.67 ± 0.14

(8/8)

4.47 ± 0.43

(7/8)

F18 fimbriae (Fed A)

8.51 ± 0.17

(14/14)

8.39 ± 0.22

(14/14)

5.03 ± 0.09

(8/8)

5.07 ± 0.08

(8/8)

4.89 ± 0.09

(8/8)

5.04 ± 0.14

(8/8)

F6 fimbriae (Fas A)*

6.60 ± 0.06

(14/14)

7.81 ± 0.12

(14/14)

4.43 ± 0.10

(8/8)

3.90 ± 0.09

(8/8)

4.28 ± 0.10

(5/8)

4.25 ± 0.12

(7/8)

K88 fimbriae (fae G)

7.15 ± 0.07

(14/14)

7.51 ± 0.14

(14/14)

5.74 + 0.07

(8/8)

4.10 ± 0.23

(8/8)

5.52 ± 0.05

(8/8)

3.60 ± 0.18

(6/8)

ETEC ^C

N/A

7.99 ± 0.17^a

(14/14)

N/A

4.63 ± 0.06^b

(8/8)

N/A

4.68 ± 0.13^b

(8/8)

Universal stress protein A

9.21 ± 0.17

(14/14)

N/A

8.92 ± 0.20

(8/8)

N/A

8.80 ± 0.19

(8/8)

N/A

Heat-labile toxin

4.24 ± 0.23^a

(9/14)

N/A

< 3^b

N/A

< 3^b

N/A

Heat-stable toxins a

6.22 ± 0.21^a

(14/14)

N/A

5.11 ± 0.71^a

(8/8)

N/A

3.02 ± 0.01^b

(8/8)

N/A

Heat-stable toxins b

7.80 ± 0.18^a

(14/14)

N/A

5.03 ± 0.09^b

(8/8)

N/A

4.89 ± 0.09^b

(8/8)

N/A