Table 1 Synthetic DNA probes as positive controls for K99, F41, F18, and F6 fimbriae gene sequences for qPCR and multiplex HRM-qPCR analysis
Target (gene bank accesson No) | Target gene | Sequence | Location | Size (bp) |
|---|---|---|---|---|
K99 fimbriae (X05797.1) | E. coli genes fanA and fanB involved in biogenesis of K99 fimbriae | GCATAAAACTCTGGTTCTTCTTGGCTGTTTATTTTTTTTTTCTATATGTTCAGTGTGTTATTTATACTCTTCCCTTTATTTTTGTTTTTTTTATGCCATATAATTCAATCAGCAGAGATGATTGGGATCATAAAAATGTCACTTGAGGGTATATGCGATCTTTTAATAAAGATGAATACTTGTTCAGGGAGAAACTTGGTTATCTTGTGAAAGGAATGGTTAAAGCAAGGTGCTTCCAATTATTAGTGGAGTTATCAAGTATACGTAGTTCTAGGG | 314–589 | 276 |
F41 fimbriae (X14354.1) | E. coli fimbriae F41a gene for F41 fimbriae subunit | ACAATTGGGATGACCTCAGTCACAGCAACTATACTTCTGCAAATAAGGCATCTTATCTCTCTTATGGATCTGGTGTTTCTGCAGGTAGTACTTTAGTTATGAATTTAAATAAGGATGTTGCGGGTCGACTTGAATGGGTGG | 916–1056 | 141 |
F18 fimbriae (KM260195.1) | E. coli isolate HDG_U113 FedA precursor (fedA) gene | AACACAGGGGCAGGAGGTTAAGGCGTCGAATAGCACTGTAAGTTTCGATGCATCAAAAGCAACTACGGAAGGTTTCAAATTTACTGCTCAACTGAAAGGTGGTCAAACCCCGGGTGACTTCCAGGGGGCAGCGGCTTACGCGGTTACTTACAAG | 357–510 | 154 |
F6 (987P) fimbriae (M35257.1) | E. coli fimbriae 987P subunit gene | ACTAAATATTTAGTTCCAGCCTCCAATGATACTAGTGCATCAGGAGTTGGCGTATACATTCAGGACAACAACGCCCAGGCTGTGGAAATTGGTACTGAAAAAACTGTACCTGTGGTATCAAATGGCGGATTAGCTCTTTCAGACCAAAGTATTCCACTGCAAGCATACATCGGAACCACCACAGGGAATCCTGA | 574–767 | 194 |