Fig. 2From: Identification and quantification of virulence factors of enterotoxigenic Escherichia coli by high-resolution melting curve quantitative PCRCalibration of F6 by changing the DNA range from 2.44 to 60% in the total gene copy number. Gene copy number of K99, F41, F18 and K88 was constant at 1× 10 10. The percentages of F6 melting peak area and DNA range were plotted to establish the calibration equation parametersBack to article page