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Table 4 Intra assay variation

From: Simultaneous detection of chikungunya virus, dengue virus and human pathogenic Leptospira genomes using a multiplex TaqMan® assay

  N Mean SD CV (%)
CHIKV
 Level 1 15 22.56 0.42 1.9
 Level 2 15 29.24 0.44 1.5
DENV
 Level 1 15 28.48 0.36 1.3
 Level 2 15 35.71 0.75 2.1
LEPTOSPIRA
 Level 1 15 21.74 0.97 4.4
 Level 2 15 28.13 0.54 1.9
  1. Level 1 accounts for medium amount of target, level 2 for low amounts of target
  2. A threshold of 5% maximum for CV was required for assay validation