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Table 4 Intra assay variation

From: Simultaneous detection of chikungunya virus, dengue virus and human pathogenic Leptospira genomes using a multiplex TaqMan® assay

 

N

Mean

SD

CV (%)

CHIKV

 Level 1

15

22.56

0.42

1.9

 Level 2

15

29.24

0.44

1.5

DENV

 Level 1

15

28.48

0.36

1.3

 Level 2

15

35.71

0.75

2.1

LEPTOSPIRA

 Level 1

15

21.74

0.97

4.4

 Level 2

15

28.13

0.54

1.9

  1. Level 1 accounts for medium amount of target, level 2 for low amounts of target
  2. A threshold of 5% maximum for CV was required for assay validation