Table 2 Analysis of discrepant results in FilmArray™ GI positive samples
From: FilmArray™ GI panel performance for the diagnosis of acute gastroenteritis or hemorragic diarrhea
# | Lab | Cat. | Laboratory test resultsa | FilmArray™ resultsa | Discrepancy analysis assays | Case resolution | Interpretation of discordance | Final analysis of FilmArray™ results |
|---|---|---|---|---|---|---|---|---|
1 | A | AGE | N | Shig /EIEC, ETEC | BD MAX™ Enteric Bacterial Panel (shig/EIEC) | Shig/EIEC, ETEC | TP | + |
2 | A | AGE | N | C. difficile | Xpert® C. difficile/Epi (real-time PCR) | C. difficile. | TP | + |
3 | A | AGE | rotavirus | rotavirus, C. difficile | Xpert® C. difficile/Epi (real-time PCR) | rotavirus, C. difficile | TP | + |
4 | A | AGE | N | rotavirus | real-time RT-PCR | rotavirus | TP | + |
5 | A | AGE | N | C. difficile | Xpert® C. difficile/Epi (real-time PCR) | C. difficile | TP | + |
6 | A | AGE | G. lamblia | G. lamblia , shig/ EIEC, EAEC | BD MAX™ Enteric Bacterial Panel (shig/EIEC) | G. lamblia, shig/EIEC, EAEC | TP | + |
7 | A | AGE | N | rotavirus | real-time RT-PCR | N | TN | FP (rotavirus) |
8 | A | AGE | N | rotavirus | real-time RT-PCR | N | TN | FP (rotavirus) |
9 | A | AGE | N | cryptosporidium | none | NA | NA | NA |
10 | A | AGE | C. difficile | C. difficile , astrovirus | Real-time RT-PCR | C. difficile | TP | + |
11 | A | AGE | N | astrovirus | Real-time RT-PCR | N | TP | + |
12 | A | AGE | C. difficile, rotavirus | C. difficile, rotavirus, EPEC | NA | C. difficile, rotavirus | NA (EPEC) | NA |
13 | B | AGE | Camp. spp., rotavirus | Camp. spp., rotavirus, shig/ EIEC | BD MAX™ Enteric Bacterial Panel | Camp. spp., rotavirus, Shig/EIEC | TP | + |
14 | B | AGE | adenovirus | adenovirus, sapovirus | Allplex™ GI assay | adenovirus, sapovirus | TP | + |
15 | B | AGE | norovirus | norovirus, salmonella , rotavirus | BD MAX™ Enteric Bacterial Panel (Salmonella) Allplex™ GI assay (rotavirus) | norovirus, Salmonella spp | TP (Salmonella spp) TN | + FP (rotavirus) |
16 | B | HD | Salmonella spp. | Salmonella spp., EPEC, norovirus | Allplex™ GI assay | Salmonella spp., norovirus | NA (EPEC) TP (norovirus) | NA + |
17 | B | HD | Camp. spp | Camp. spp., EPEC | None | Camp. spp | NA (EPEC) | NA |
18 | B | HD | N | rotavirus | real-time PCR | N | TP | + |
19 | B | HD | N | rotavirus | real-time PCR | N | TP | + |
20 | B | HD | N | EAEC | none | NA | NA | NA |
21 | B | HD | Salmonella spp. | Salmonella spp., astrovirus, EAEC | Allplex™ GI assay (astrovirus) none (EAEC) | Salmonella spp | TN NA (EAEC) | FP (astrovirus) NA |
22 | B | HD | EHEC, EAEC, ETEC, EPEC | STEC, EAEC, ETEC, sapovirus | Allplex™ GI assay (sapovirus) none (EPEC) | STEC, EAEA | TN (sapovirus) NA | FP (sapovirus) NA |
23 | B | AGE | rotavirus, adenovirus | rotavirus | PCR and sequencing | rotavirus, adenovirus (AdV-C1) | TP (adenovirus) | +b (adenovirus) |
24 | B | HD | Camp. spp., Shigella spp | Camp. spp | BD MAX™ Enteric Bacterial Panel | Camp. spp | TN | + |
25 | B | HD | Camp. spp., EPEC | Camp. spp | none | Camp. spp | NA | NA |
26 | B | HD | Camp. spp., Shigella spp | Camp. spp | BD MAX™ Enteric Bacterial Panel | Camp. spp | TN | + |
27 | B | HD | Camp. spp., Shigella spp | Camp. spp | BD MAX™ Enteric Bacterial Panel | Camp. spp | TN | + |
28 | B | HD | STEC, Y. enterocolitica, C. difficile | STEC, Y. enterocolitica | BD MAX™ Enteric Bacterial Panel | STEC, Y. enterocolitica | TN (C. difficile) | + |
29 | B | HD | STEC, EPEC | STEC | none | STEC | NA (EPEC) | + |
30 | A | AGE | norovirus, C. difficile | norovirus, EPEC | Xpert® C. difficile/Epi (real-time PCR) NA (EPEC) | norovirus | TN (C. difficile) NA (EPEC) | + NA |
31 | A | AGE | C. difficile | Camp. spp | Xpert® C. difficile/Epi (real-time PCR) BD MAX™ Enteric Bacterial Panel (Camp spp) | Camp. spp | TN (C. difficile) TP (Camp. spp.) | + + |
32 | A | AGE | rotavirus | Camp. spp | real-time PCR (rotavirus) BD MAX™ Enteric Bacterial Panel (Camp spp) | Camp. spp | TN (rotavirus) TP (Camp. spp.) | + + |
33 | A | AGE | rotavirus, adenovirus | rotavirus, EPEC | PCR/sequencing (adenovirus) EPEC (NA) | adenovirus (AdV-A12) | TN (adenovirus)b NA(EPEC) | +b NA |
34 | B | AGE | norovirus, salmonella spp., adenovirus | norovirus, salmonella spp., rotavirus | Allplex™ GI assay | norovirus, salmonella spp | TN (adenovirus) TN | + FP (rotavirus) |
35 | B | AGE | norovirus | sapovirus | Allplex™ GI assay | sapovirus | TP (sapovirus) TN (norovirus) | + + |
36 | B | AGE | adenovirs | Shig/EIEC | BD MAX™ Enteric Bacterial Panel (Shig/EIEC) Allplex™ GI assay (adenovirus) | adenovirus (AdV-C1) | TN (Shig/EIEC) TP (adenovirus)b | FP (shig/EIEC) +b (adenovirus) |
37 | B | HD | sapovirus, EPEC, STEC | sapovirus, EPEC, Camp. spp. , | BD MAX™ Enteric Bacterial Panel | Camp. spp | TN (STEC) TP (Camp. spp.) | + + |
38 | B | HD | C. difficile | Salmonella spp | BD MAX™ Enteric Bacterial Panel | Salmonella spp | TN (C. difficile) TP (Salmonella spp) | + + |
39 | B | HD | C. difficile | Salmonella spp., rotavirus, norovirus | BD MAX™ Enteric Bacterial Panel | Negative with confirmed test | NA | NA |
40 | B | HD | EPEC | STEC , G. lamblia | BD MAX™ Enteric Bacterial Panel | STEC, G. lamblia | TP (Stx1/2) TP (G. lamblia) | + + |
41 | B | HD | E. coli O157 | EPEC, ETEC | BD MAX™ Enteric Bacterial Panel | negative | NA | NA |
42 | B | HD | EAEC | STEC | BD MAX™ Enteric Bacterial Panel | STEC | TP (STEC) NA (EAEC) | + NA |