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Fig. 3 | BMC Microbiology

Fig. 3

From: The LspC3–41I restriction-modification system is the major determinant for genetic manipulations of Lysinibacillus sphaericus C3–41

Fig. 3

The effect of Bsph_0498 (encoding LspC3–41I) on the restriction role of L. sphaericus C3–41 CFE. Untreated and pre-treated plasmid pBU4 was incubated with CFE and then subjected to restriction assays, and the reaction mixture was analyzed by agarose gel electrophoresis as show in the left three pictures (L) and Southern blot analysis as show in the right three pictures (R). a untreated pBU4. Lane 1, C3–41; lane 2, Δ0498; lane 3, RC0498; lane 4, HaeIII digested; lane 5, Untreated. b pBU4 methylated with C3–41 CFE. Lane 1, C3–41; lane 2, Δ0498; lane 3, RC0498; lane 4, HaeIII digested; lane 5, Untreated. c pBU4 methylated with MTase M. HaeIII. Lane 1, C3–41; lane 2, Δ0498; lane 3, RC0498; lane 4, HaeIII digested; lane 5, Untreated. M: DNA marker

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