Skip to main content
Fig. 2 | BMC Microbiology

Fig. 2

From: The LspC3–41I restriction-modification system is the major determinant for genetic manipulations of Lysinibacillus sphaericus C3–41

Fig. 2

The restriction and methylation role of L. sphaericus C3–41 CFE. a Restriction profile of pBU4 (a) and Southern blot analysis of the restriction of pBU4 using linear pBU4 as the probe (b). Lane 1, pBU4 mock treated; lane 2, pBU4 digested by HaeIII; lane 3, pBU4 digested by C3–41 CFE; lane 4, pBU4 methylated with C3–41 CFE; lane 5, pBU4 methylated with C3–41 CFE and then digested by HaeIII. b Restriction assays of L. sphaericus C3–41 chromosome. Lane 1, chromosome mock treated; lane 2, chromosome digested by HaeIII; lane 3, chromosome digested by HindIII. M: DNA marker

Back to article page