Table 2 Primers used in this study
Oligonucleotides | Sequence |
|---|---|
Glms-F | CCCCCCCGAGCTCAGGTTACCCGGATCTATGGAAATCGGCGTAGCGTCAACCAAG |
Glms-R | CCCTCGAGTACGCGTCACTAGTGGGGCCCTTCGCGCTTTTATTCTACGGTAACCG |
Glms-yz-F | AGAGATTGGCTACTTGGGATCGTTG |
KmCS-F (SalI) | ACGCGTCGACATTGTGAGCGGATAACAATTTGTGG |
KmCS-R (SalI) | ACGCGTCGACTAGATCCGGGTAACCTGAGCT |
evpP-F | TCATCGCACATACAGAATAAACGCC |
evpP-R | CCGTAACATTTCTTACAACACTGCG |
virB9-P1 | CCCCCCCGAGCTCAGGTTACCCGGATCTATGGCCACTGGTTTGTTGTAGGGCCAT |
virB9-P2 | CAAATTGCCATGGGCTGATGGCTGAGAACAGAGACGATCTGGA |
virB9-P3 | TGTTCTCAGCCATCAGCCCATGGCAATTTGTTGGACCGTT |
virB9-P4 | GAGTACGCGTCACTAGTGGGGCCCTTCTAGCGCGAGGGCTATCAGTGGGAAACCC |
virB9-out-F | GCGCCCAGGCCGTCCGCTCGTTCAG |
virB9-out-R | CCGCGTCGATAACAACACTGGCGTG |
virB9-in-F | TTGACTCCCTCTAATTACTCGCTCA |
virB9-in-R | GTCGGAAATCACATTTTCATCAAGC |
p007-P1 | CCCCCCCGAGCTCAGGTTACCCGGATCTATTGGCGCGGGTCGGTATATGCGGCAT |
p007-P2 | AGGAGAGTTCGAGTGGCTTGGTCATCCGAGGAATGGAGGC |
p007-P3 | CTCGGATGACCAAGCCACTCGAACTCTCCTTGATCAGTGT |
p007-P4 | GAGTACGCGTCACTAGTGGGGCCCTTCTAGTTTTTGAAAGCTGGCTAGGCATGGT |
p007-out-F | CTGCGCTCCCCTGCCCTTTTCACCT |
p007-out-R | TGCGCTTTCTCTCGTTGTGGCGTTC |
topA-P1 | CCCCCCCGAGCTCAGGTTACCCGGATCTATGGTCCAGGACCCAATCCACCCCTTC |
topA-P2 | TAGGGAGGACCAATGTGAGCTTGGCGGCAATCAAAGTTGT |
topA-P3 | TTGCCGCCAAGCTCACATTGGTCCTCCCTACCGTCAACCA |
topA-P4 | GAGTACGCGTCACTAGTGGGGCCCTTCTAGACCTCCAGTCGGTCGAGTTGAGCAA |
topA-out-F | GGCCGCGATAATCAGGTCAACGATG |
topA-out-R | CACCTGTGCGGCCCTGTCCGGGGCT |
topA-in-F | ACCAAAAATCGTAACCCTTCTTGCC |
topA-in-R | AGGGTGGGACGTGCAGGCCAGTGTC |
pDMK-PF | AAAGCTCTCATCAACCGTGGC |
pDMK-PR | TGCTCCAGTGGCTTCTGTTTC |
topA-C-F | GATCCTCTAGATTTAAGAAGGAGATATACAATGAATCTAGTTATTGTTGA |
topA-C-R | GATCCCCCGGGCTGCAGGAATTCGATATCATCAAATCTTTGGCTTGCCAC |
p007-C-F | GATCCTCTAGATTTAAGAAGGAGATATACAGTGAACCTGAAAACACTAAG |
p007-C-R | GATCCCCCGGGCTGCAGGAATTCGATATCATCAGTGATGGCCTCCATTCC |
virB9-C-F | GATCCTCTAGATTTAAGAAGGAGATATACAATGATACGAGCAAAATCACT |
virB9-C-R | GATCCCCCGGGCTGCAGGAATTCGATATCATCATTGACTCCCTCTAATTA |
virB9-qRT-F | TCATGTTCGTGGTCGCATCA |
virB9-qRT-R | CCATTTTGGCTTCTCCACGC |
virB4-qRT-F | TGGGGGCCGTTTTGAGATAC |
virB4-qRT-R | GCGGCAGCTTCAATAACCAG |
avtA-qRT-F | TTACTCCGCAATCACCCGTC |
avtA-qRT-R | CAGCTCACCGCATAGGGAG |
P021-qRT-F | AAAGCCCCAAACCGTAAAGC |
P021-qRT-R | ATGCGGGAATGGGTCAGTTT |
gyrB-qRT-F | CCGATGATGGTACGGGTCTG |
gyrB-qRT-R | GCTTTTCAGACAGGGCGTTC |
p003-qRT-F | GCCGAAGCGTTCCCAAAAAT |
p003-qRT-R | CCTGTGGAATCGCATCGAGA |
pD43B-p003-F | GAGCTCAGGTTACCCGCATGCAAGATCTATATGATGGCTGAGAACAGAGACGATC |
pD43B-p003-R | ATACGTATTTGACATTCAATCGGCTTTGAGGTCATATACC |
pD43B-gyrB-F | CTCAAAGCCGATTGAATGTCAAATACGTATGACTCCTCAA |
pD43B-gyrB-R | CCCTCGAGTACGCGTCACTAGTGGGGCCCTTTAAAAGTCCAGATTGGACGCTTTA |