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Table 7 Bacterial strains and plasmids used in this study

From: DFI-seq identification of environment-specific gene expression in uropathogenic Escherichia coli

Strain Genotype Source
 UTI89 Serotype O18:K1:H7 [5] Provided by Emile Van Schaftingen
 UTI89/pMW82 AmpR This study
 UTI89/pMWLib AmpR This study
 DH5α F– Φ80lacZΔM15 Δ(lacZYA-argF) U169 recA1 endA1 hsdR17 (rK–, mK+) phoA supE44 λ– thi-1 gyrA96 relA1 Invitrogen
 UTI89ΔargC ΔargC, CmlR This study
 UTI89ΔargE ΔargE, CmlR This study
 UTI89ΔargG ΔargG, CmlR This study
 UTI89ΔartJ ΔartJ, CmlR This study
 UTI89ΔilvG ΔilvG, CmlR This study
 UTI89ΔmetA ΔmetA, CmlR This study
 UTI89ΔmetE ΔmetE, CmlR This study
 UTI89ΔmetF ΔmetF, CmlR This study
 UTI89ΔpotF ΔpotF, CmlR This study
 UTI89ΔybdH ΔybdH, CmlR This study
 UTI89ΔybdL ΔybdL, CmlR This study
 UTI89ΔyibI ΔyibI, CmlR This study
 UTI89ΔargA ΔargA, CmlR This study
 UTI89ΔserA ΔserA, CmlR This study
 UTI89ΔyeaR ΔyeaR, CmlR This study
 UTI89ΔargB ΔargB, CmlR This study
 UTI89ΔmetR ΔmetR, CmlR This study
 UTI89ΔyjaB ΔyjaB, CmlR This study
 UTI89ΔC5139 ΔC5139, KanR This study
Plasmid Genotype Source
 pKD3 Cml template plasmid [28]
 pKD4 Kan template plasmid [28]
 pKD46 Bla, λ Red recombinase expression plasmid [28]
 pMW82   [22] Kindly provided by Dirk Bumann
 pMWLib pMW82 containing random 500−700 bp long UTI89 DNA segments