Table 7 Bacterial strains and plasmids used in this study

Strain

Genotype

Source

 UTI89

Serotype O18:K1:H7

[5] Provided by Emile Van Schaftingen

 UTI89/pMW82

Amp^R

This study

 UTI89/pMWLib

Amp^R

This study

 DH5α

F– Φ80lacZΔM15 Δ(lacZYA-argF) U169 recA1 endA1 hsdR17 (rK–, mK+) phoA supE44 λ– thi-1 gyrA96 relA1

Invitrogen

 UTI89ΔargC

ΔargC, Cml^R

This study

 UTI89ΔargE

ΔargE, Cml^R

This study

 UTI89ΔargG

ΔargG, Cml^R

This study

 UTI89ΔartJ

ΔartJ, Cml^R

This study

 UTI89ΔilvG

ΔilvG, Cml^R

This study

 UTI89ΔmetA

ΔmetA, Cml^R

This study

 UTI89ΔmetE

ΔmetE, Cml^R

This study

 UTI89ΔmetF

ΔmetF, Cml^R

This study

 UTI89ΔpotF

ΔpotF, Cml^R

This study

 UTI89ΔybdH

ΔybdH, Cml^R

This study

 UTI89ΔybdL

ΔybdL, Cml^R

This study

 UTI89ΔyibI

ΔyibI, Cml^R

This study

 UTI89ΔargA

ΔargA, Cml^R

This study

 UTI89ΔserA

ΔserA, Cml^R

This study

 UTI89ΔyeaR

ΔyeaR, Cml^R

This study

 UTI89ΔargB

ΔargB, Cml^R

This study

 UTI89ΔmetR

ΔmetR, Cml^R

This study

 UTI89ΔyjaB

ΔyjaB, Cml^R

This study

 UTI89ΔC5139

ΔC5139, Kan^R

This study

Plasmid

Genotype

Source

 pKD3

Cml template plasmid

[28]

 pKD4

Kan template plasmid

[28]

 pKD46

Bla, λ Red recombinase expression plasmid

[28]

 pMW82

[22] Kindly provided by Dirk Bumann

 pMWLib

pMW82 containing random 500−700 bp long UTI89 DNA segments