Table 1 DFI sorting overview. Proportion of population enriched by sorting. Sort number 1, 3, and 5 enrich for green fluorescent bacteria; sort number 2 and 4 enrich for non-fluorescent bacteria
From: DFI-seq identification of environment-specific gene expression in uropathogenic Escherichia coli
Sort number | Original bacterial culture | Growth medium | Proportion of population enriched by sorting |
|---|---|---|---|
1 | Original UTI89/pMWLib | Human urine | 2% (n = 307,176) |
2 | Fluorescent bacteria isolated during the 1. Sort | LB | 20.5% (n = 1,249,036) |
3 | Non-fluorescent bacteria isolated during the 2. Sort | Human urine | 7.1% (n = 401,299) |
4 | Fluorescent bacteria isolated during the 3. Sort | LB | 74.8% (n = 1,733,294) |
5 | Non-fluorescent bacteria isolated during the 4. Sort | Human urine | 60.9% (n = 409,343) |