Fig. 1
From: DFI-seq identification of environment-specific gene expression in uropathogenic Escherichia coli
A promoter trap library is created using a gfp gene as reporter. The library is transformed into the bacteria of interest. The pool of transformants is both used to infect a tissue culture model and grown in vitro. From both environments, green fluorescent bacteria are enriched using a fluorescence-activated cell sorting instrument and grown over night in vitro. From the two populations plasmids are purified and PCR is performed to amplify trapped promoters. Through next-generation sequencing, host-induced promoters can be identified by comparing promoter read counts between the two conditions