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Table 1 Amylase binding by streptococcus strains in our collection

From: Comparative genomics and evolution of the amylase-binding proteins of oral streptococci

Streptococcus species Strain designation Amylase ligand-binding overlaya (ca. mw kDa) Amylase-binding activityb GenBank accession number
S. anginosus ATCC 33397 (NCTC 10713) - -  
  UC2953 - ND  
  UC9218 - ND  
  NCTC 10708c - -  
  NCTC 10709c - -  
S. australis ATCC 700641 22, 35, 86 + NZ_AFUD01000015
S. cristatus CC5A 26, 26, 84 + NZ_JYGJ00000000d.j
  CR3 28, 28, 84 + NZ_JYGK00000000d,j
  CR311 (ATCC 51100) 30, 82e + NZ_AFUE01000002
S. gordonii ATCC 10558 (NCTC 7865) 20, 82e ND  
  Blackburn (NCTC 10231) 20, 82e ND  
  Challis CH1 20, 82e + NC_009785
  I141i 20, 84 + NZ_JYOZ00000000d
  CN2814 20, 82e ND  
  FAS4 20, 82e ND  
  GEO2 20, 82e ND  
  G9B 20, 82e + NZ_JYGL00000000d,j
  UB10712f,i 20, 37, 82e + NZ_JYGN00000000d
  JF2 20, 82e   
  LGR2 20, 82e ND  
  MJ2 20, 82e ND  
  M5 20, 82e ND  
  SPED3 20, 82e ND  
S. infantis ATCC 700779 - - PRJNA158721
  UC921Ai 26, 30 + NZ_JYGT00000000d
  UC6950Ai 30 + NZ_JYOV00000000d
S. intermedius ATCC 27335 (SK54) - - PRJNA197004
S. mitis NS51/SD142 36 g + EF989012.1
(ATCC 49456)    
(NCTC 12261)    
  OT25 36 + NZ_JYGP00000000d, j
  SK137 36, 50, 63 + NZ_JYGQ00000000d,j
  SK145 37, 48, 65 + NZ_JYGS00000000d,j
  UC2948 - -  
  UC3161 - -  
S. mutans NCTC 10449 (ATCC 25175) - -  
  BM71 - ND  
  GS5 - ND  
  Ingbritt - -  
  LT11 - ND  
  OMZ175 - ND  
  NG8 - ND  
  VT321 - ND  
  V202 - ND  
S. oralis     
ssp oralis COL85/1862i 26, 29 + NZ_JYGM00000000d
ssp oralis OP51i 25, 26, 30 + NZ_JYGO00000000d
ssp oralis SK141h,i 26, 26, 30 + NZ_JYGR00000000d
ssp tigurinus UC5873i 26, 30 + NZ_JYGU00000000d
  ATCC 10557 (NCTC 7864) - -  
  BU174 -e ND  
  C104 - ND  
  CR834 -e ND  
  KS32AR - ND  
  MPD1 -e ND  
  SK92 - -  
  34 - ND  
  9811 -e ND  
  16532AR - ND  
S. parasanguinis MGH413 21, 87 + NZ_JYOW00000000d,j
  VT517i 22, 84 + NZ_JYPA00000000d
S. salivarius KB005 29 + NZ_JYOX00000000d,j
  CDC013 29 ND  
  CDC017 29 ND  
  CM103 29 ND  
  MG568 29 ND  
  MG587 29, 51 ND  
  MG691 29, 49 ND  
  UC810 29 ND  
  UC3162 29, 50 + NZ_JYOY00000000d
  TOUR 29 ND  
  13,419-R 29 ND  
S. sanguinis ATCC 10556 (NCTC 7863) - -  
  HPC1 - ND  
  MPC1 -e ND  
  UC9433 - -  
  804 - ND  
S. sobrinus B13 - ND  
  SL1 - -  
  K1R - ND  
  OMZ 176 - ND  
  6715-WT13 - ND  
  1. ND not done
  2. aAmylase-ligand binding assay: detects the ability of proteins from 20× concentrated culture supernatant to bind salivary amylase in whole saliva by Far-Western blot (Methods)
  3. bAmylase-binding activity: measures the ability of bacterial cells to bind salivary amylase in whole saliva and reduce hydrolysis in starch agarose. (+) ABP, (-) ABP on bacterial cells (Methods)
  4. c S. milleri reclassified as S. anginosis in Ruoff et al. [48]
  5. dSabharwal et al. [22]; strains sequenced for this study
  6. eBrown et al. [3]
  7. fStrain labeled NCTC 10712 (S. mitis) in our collection is now corrected to S. gordonii, UB10712 upon whole genome sequencing
  8. gVorrasi et al. [16]
  9. hThe strain from our collection named S. mitis in Brown et al. [3] Submitted to GenBank as S. mitis; updated to S. oralis in 2015 as per reclassification Takenouchi-Ohkubo et al. [28] and Kilian et al. [49]
  10. iUpdated taxonomic classifications for Mitis group, Jensen et al. [23]
  11. jReference strains used for genome post-assembly: S. cristatus ATCC 51100 for S. cristatus CC5A and CR3; S. gordonii Challis substrain CH1 for S. gordonii G9B; S. mitis B6 for S. mitis OT25, SK145, SK137; S. parasanguinis ATCC 15912 for S. parasanguinis MGH413; S. salivarius CCHSS3 for S. salivarius KB005