Comparative genomics and evolution of the amylase-binding proteins of oral streptococci

Haase, Elaine M.; Kou, Yurong; Sabharwal, Amarpreet; Liao, Yu-Chieh; Lan, Tianying; Lindqvist, Charlotte; Scannapieco, Frank A.

doi:10.1186/s12866-017-1005-7

BMC Microbiology

Table 1 Amylase binding by streptococcus strains in our collection

From: Comparative genomics and evolution of the amylase-binding proteins of oral streptococci

Streptococcus species	Strain designation	Amylase ligand-binding overlay^a (ca. mw kDa)	Amylase-binding activity^b	GenBank accession number
S. anginosus	ATCC 33397 (NCTC 10713)	-	-
	UC2953	-	ND
	UC9218	-	ND
	NCTC 10708^c	-	-
	NCTC 10709^c	-	-
S. australis	ATCC 700641	22, 35, 86	+	NZ_AFUD01000015
S. cristatus	CC5A	26, 26, 84	+	NZ_JYGJ00000000^d.j
	CR3	28, 28, 84	+	NZ_JYGK00000000^d,j
	CR311 (ATCC 51100)	30, 82^e	+	NZ_AFUE01000002
S. gordonii	ATCC 10558 (NCTC 7865)	20, 82^e	ND
	Blackburn (NCTC 10231)	20, 82^e	ND
	Challis CH1	20, 82^e	+	NC_009785
	I141ⁱ	20, 84	+	NZ_JYOZ00000000^d
	CN2814	20, 82^e	ND
	FAS4	20, 82^e	ND
	GEO2	20, 82^e	ND
	G9B	20, 82^e	+	NZ_JYGL00000000^d,j
	UB10712^f,i	20, 37, 82^e	+	NZ_JYGN00000000^d
	JF2	20, 82^e
	LGR2	20, 82^e	ND
	MJ2	20, 82^e	ND
	M5	20, 82^e	ND
	SPED3	20, 82^e	ND
S. infantis	ATCC 700779	-	-	PRJNA158721
	UC921Aⁱ	26, 30	+	NZ_JYGT00000000^d
	UC6950Aⁱ	30	+	NZ_JYOV00000000^d
S. intermedius	ATCC 27335 (SK54)	-	-	PRJNA197004
S. mitis	NS51/SD142	36 ^g	+	EF989012.1
	(ATCC 49456)
	(NCTC 12261)
	OT25	36	+	NZ_JYGP00000000^{d, j}
	SK137	36, 50, 63	+	NZ_JYGQ00000000^d,j
	SK145	37, 48, 65	+	NZ_JYGS00000000^d,j
	UC2948	-	-
	UC3161	-	-
S. mutans	NCTC 10449 (ATCC 25175)	-	-
	BM71	-	ND
	GS5	-	ND
	Ingbritt	-	-
	LT11	-	ND
	OMZ175	-	ND
	NG8	-	ND
	VT321	-	ND
	V202	-	ND
S. oralis
ssp oralis	COL85/1862ⁱ	26, 29	+	NZ_JYGM00000000^d
ssp oralis	OP51ⁱ	25, 26, 30	+	NZ_JYGO00000000^d
ssp oralis	SK141^h,i	26, 26, 30	+	NZ_JYGR00000000^d
ssp tigurinus	UC5873ⁱ	26, 30	+	NZ_JYGU00000000^d
	ATCC 10557 (NCTC 7864)	-	-
	BU174	-^e	ND
	C104	-	ND
	CR834	-^e	ND
	KS32AR	-	ND
	MPD1	-^e	ND
	SK92	-	-
	34	-	ND
	9811	-^e	ND
	16532AR	-	ND
S. parasanguinis	MGH413	21, 87	+	NZ_JYOW00000000^d,j
	VT517ⁱ	22, 84	+	NZ_JYPA00000000^d
S. salivarius	KB005	29	+	NZ_JYOX00000000^d,j
	CDC013	29	ND
	CDC017	29	ND
	CM103	29	ND
	MG568	29	ND
	MG587	29, 51	ND
	MG691	29, 49	ND
	UC810	29	ND
	UC3162	29, 50	+	NZ_JYOY00000000^d
	TOUR	29	ND
	13,419-R	29	ND
S. sanguinis	ATCC 10556 (NCTC 7863)	-	-
	HPC1	-	ND
	MPC1	-^e	ND
	UC9433	-	-
	804	-	ND
S. sobrinus	B13	-	ND
	SL1	-	-
	K1R	-	ND
	OMZ 176	-	ND
	6715-WT13	-	ND

ND not done
^aAmylase-ligand binding assay: detects the ability of proteins from 20× concentrated culture supernatant to bind salivary amylase in whole saliva by Far-Western blot (Methods)
^bAmylase-binding activity: measures the ability of bacterial cells to bind salivary amylase in whole saliva and reduce hydrolysis in starch agarose. (+) ABP, (-) ABP on bacterial cells (Methods)
^c S. milleri reclassified as S. anginosis in Ruoff et al. [48]
^dSabharwal et al. [22]; strains sequenced for this study
^eBrown et al. [3]
^fStrain labeled NCTC 10712 (S. mitis) in our collection is now corrected to S. gordonii, UB10712 upon whole genome sequencing
^gVorrasi et al. [16]
^hThe strain from our collection named S. mitis in Brown et al. [3] Submitted to GenBank as S. mitis; updated to S. oralis in 2015 as per reclassification Takenouchi-Ohkubo et al. [28] and Kilian et al. [49]
ⁱUpdated taxonomic classifications for Mitis group, Jensen et al. [23]
^jReference strains used for genome post-assembly: S. cristatus ATCC 51100 for S. cristatus CC5A and CR3; S. gordonii Challis substrain CH1 for S. gordonii G9B; S. mitis B6 for S. mitis OT25, SK145, SK137; S. parasanguinis ATCC 15912 for S. parasanguinis MGH413; S. salivarius CCHSS3 for S. salivarius KB005

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