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Fig. 6 | BMC Microbiology

Fig. 6

From: Comparative proteomic analysis of Neisseria meningitidis wildtype and dprA null mutant strains links DNA processing to pilus biogenesis

Fig. 6

PilG and DprA immunoblots. MC58 wt and ΔdprA mutant overnight cultures were harvested in 1× PBS and heat inactivated at 600C for 30 min. The cells were disrupted using MagNa Lyser (6× 90s at a speed of 6000 rpm). The cell lysates were prepared in Laemmli sample buffer and SDS-PAGE was run. The proteins transferred onto PVDF membrane. The membranes were incubated with ati-PilG (a) and ati-DprA (b) rabbit polyclonal primary antibodies, and anti-rabbit-IgG-horseradish peroxidase conjugate secondary antibody. The immunoblots were developed with Immun-Star WesternC Chemiluminescent kit (Bio-Rad) and visualized using a ChemiDoc Touch imager (Bio-Rad). Results were analysed using the Image Lab software (Bio-Rad)

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BMC Microbiology

ISSN: 1471-2180

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