Fig. 3

Activation of c-Jun through Src signaling is triggered in macrophages upon S. aureus infection. a The activation of the Src-JNK-c-Jun, MEK-Erk, and p38 signaling pathways was investigated by determining the expression levels of phosphorylated Src, c-Jun, p38, and Erk, respectively, by Western blotting. Samples were collected 1 h post-infection. b The relative densitometric quantification of above results is shown to indicate the fold change of protein levels relative to untreated cells after normalization to GAPDH. * P < 0.05