Fig. 7From: Localization of Staphylococcus aureus in tissue from the nasal vestibule in healthy carriersIntracellular localization of S. aureus in nasal epithelial cells. S. aureus is labeled with primary rabbit polyclonal antibody to S. aureus (Abcam), secondary antibody Alexa Fluor 488 goat anti-rabbit IgG (green) (Molecular Probes™, Thermo Fisher Scientific), DRAQ5 (BioStatus) for keratinocyte nuclei (blue) and Alexa Fluor 594 Phalloidin (A12381, Molecular Probes™; Thermo Fisher Scientific) for actin (red). Confocal laser scanning microscopy of frozen sections. Projection is constructed from confocal Z-stacks (0,2 um thick), 63× objective. Image to the left and on top corresponds to a vertical view in the z-plane. Z-plane images reveal a single cellular nucleus (blue) closely related to fluorescing S. aureus (green)Back to article page