Fig. 6
From: Localization of Staphylococcus aureus in tissue from the nasal vestibule in healthy carriers
Localization of S. aureus in the lower part of epidermis by CLSM. Overview and detailed image/zoomed portion of the image showing (a) S. aureus on the border between stratum spinosum and stratum basale, and (b) S. aureus on the border between the stratum basale and dermis. Inset represents a zoomed portion of the image. Scale bar is in micrometers. Green fluorescence labeling of S. aureus, blue fluorescence labeling of keratinocyte cell nuclei and S. aureus DNA. Primary antibody rabbit polyclonal antibody to S. aureus (ab20920, Abcam), secondary antibody Alexa Fluor 488® goat anti-rabbit IgG (Molecular Probe™, Thermo Fisher Scientific), and DRAQ5 (BioStatus). We used a CLSM ×63 objective