Fig. 5
From: Localization of Staphylococcus aureus in tissue from the nasal vestibule in healthy carriers
Localization of S. aureus on the border between stratum spinosum and stratum basale. Overview (a) and detailed image (b) of nasal tissue from an S. aureus carrier by confocal laser scanning microscopy. Scale bar is in micrometers. White brace indicates the region which is magnified in (b). Green fluorescence labeling of S. aureus, blue fluorescence labeling of keratinocyte cell nuclei and S. aureus DNA. Primary antibody rabbit polyclonal antibody to S. aureus (ab20920, Abcam), secondary antibody Alexa Fluor 488® goat anti-rabbit IgG (Molecular Probe™, Thermo Fisher Scientific), and DRAQ5 (BioStatus). We used a CLSM ×63 objective