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Table 1 Species discrimination by HRM analysis

From: Discrimination of three genetically close Aspergillus species by using high resolution melting analysis applied to indoor air as case study

Genus

Species

Reference

BCCM/IHEM a

Tm

mean ± SD

(°C) b

Cluster c

Confidence

mean ± SD d

(%)

Aspergillus

versicolor

IHEM 1323

76.45 ± 0.07

1

98.4 ± 0.4

Aspergillus

versicolor

IHEM 1355

76.30 ± 0.14

1

97.6 ± 2.2

Aspergillus

versicolor

IHEM 2023

76.40 ± 0.28

1

98.9 ± 0.9

Aspergillus

versicolor

IHEM 2157

76.35 ± 0.21

1

98.6 ± 0.4

Aspergillus

versicolor

IHEM 2983

76.30 ± 0.14

1

98.6 ± 1.0

Aspergillus

versicolor

IHEM 6598

76.40 ± 0.28

1

98.2 ± 0.4

Aspergillus

versicolor

IHEM 9674

76.55 ± 0.07

1

98.9 ± 0.4

Aspergillus

versicolor

IHEM 10351

76.30 ± 0.14

1

99.0 ± 0.5

Aspergillus

versicolor

IHEM 18884

76.40 ± 0.28

1

98.6 ± 0.9

Aspergillus

versicolor

IHEM 19014

76.35 ± 0.21

1

98.8 ± 0.6

Aspergillus

versicolor

IHEM 19210

76.40 ± 0.01

1

98.5 ± 0.9

Aspergillus

versicolor

IHEM 19256

76.40 ± 0.28

1

98.8 ± 0.2

Aspergillus

versicolor

IHEM 22014

76.35 ± 0.21

1

98.3 ± 1.1

Aspergillus

versicolor

IHEM 22975

76.30 ± 0.14

1

98.6 ± 1.0

Aspergillus

versicolor

IHEM 24424

76.50 ± 0.42

1

99.0 ± 0.5

Aspergillus

sydowii

IHEM 895

76.35 ± 0.21

2

99.1 ± 0.4

Aspergillus

sydowii

IHEM 1360

76.40 ± 0.00

2

99.2 ± 0.3

Aspergillus

sydowii

IHEM 20347

76.50 ± 0.14

2

99.8 ± 0.1

Aspergillus

creber

IHEM 2646

76.40 ± 0.28

3

97.9 ± 1.2

Aspergillus

creber

IHEM 2916

76.38 ± 0.08

3

98.9 ± 1.3

Penicillium

chrysogenum

IHEM 20859

/

ND

/

Penicillium

chrysogenum

IHEM 4151

/

ND

/

  1. The strains in bold are considered as a reference used for the assay development and are fully characterized as respectively A. creber, A. sydowii and A. versicolor. a Pure strains from the BCCM/IHEM collection. b Average of the Tm ± standard deviation (SD) obtained for each strain analyzed in duplicate during 2 independent runs. c Cluster 1, 2 and 3 were defined with the Biorad Precision Melt Analysis software 1.2 (Temse, Belgium). ND: not detected. d Average of the percentage of confidence (± standard deviation SD) from the mean of the cluster, defined with the Biorad Precision Melt Analysis software 1.2 (Temse, Belgium). The percentage of confidence threshold was defined as 95%, below this threshold the result is considered as not acceptable as a true positive