Skip to main content

Table 1 Species discrimination by HRM analysis

From: Discrimination of three genetically close Aspergillus species by using high resolution melting analysis applied to indoor air as case study

Genus Species Reference
BCCM/IHEM a
Tm
mean ± SD
(°C) b
Cluster c Confidence
mean ± SD d
(%)
Aspergillus versicolor IHEM 1323 76.45 ± 0.07 1 98.4 ± 0.4
Aspergillus versicolor IHEM 1355 76.30 ± 0.14 1 97.6 ± 2.2
Aspergillus versicolor IHEM 2023 76.40 ± 0.28 1 98.9 ± 0.9
Aspergillus versicolor IHEM 2157 76.35 ± 0.21 1 98.6 ± 0.4
Aspergillus versicolor IHEM 2983 76.30 ± 0.14 1 98.6 ± 1.0
Aspergillus versicolor IHEM 6598 76.40 ± 0.28 1 98.2 ± 0.4
Aspergillus versicolor IHEM 9674 76.55 ± 0.07 1 98.9 ± 0.4
Aspergillus versicolor IHEM 10351 76.30 ± 0.14 1 99.0 ± 0.5
Aspergillus versicolor IHEM 18884 76.40 ± 0.28 1 98.6 ± 0.9
Aspergillus versicolor IHEM 19014 76.35 ± 0.21 1 98.8 ± 0.6
Aspergillus versicolor IHEM 19210 76.40 ± 0.01 1 98.5 ± 0.9
Aspergillus versicolor IHEM 19256 76.40 ± 0.28 1 98.8 ± 0.2
Aspergillus versicolor IHEM 22014 76.35 ± 0.21 1 98.3 ± 1.1
Aspergillus versicolor IHEM 22975 76.30 ± 0.14 1 98.6 ± 1.0
Aspergillus versicolor IHEM 24424 76.50 ± 0.42 1 99.0 ± 0.5
Aspergillus sydowii IHEM 895 76.35 ± 0.21 2 99.1 ± 0.4
Aspergillus sydowii IHEM 1360 76.40 ± 0.00 2 99.2 ± 0.3
Aspergillus sydowii IHEM 20347 76.50 ± 0.14 2 99.8 ± 0.1
Aspergillus creber IHEM 2646 76.40 ± 0.28 3 97.9 ± 1.2
Aspergillus creber IHEM 2916 76.38 ± 0.08 3 98.9 ± 1.3
Penicillium chrysogenum IHEM 20859 / ND /
Penicillium chrysogenum IHEM 4151 / ND /
  1. The strains in bold are considered as a reference used for the assay development and are fully characterized as respectively A. creber, A. sydowii and A. versicolor. a Pure strains from the BCCM/IHEM collection. b Average of the Tm ± standard deviation (SD) obtained for each strain analyzed in duplicate during 2 independent runs. c Cluster 1, 2 and 3 were defined with the Biorad Precision Melt Analysis software 1.2 (Temse, Belgium). ND: not detected. d Average of the percentage of confidence (± standard deviation SD) from the mean of the cluster, defined with the Biorad Precision Melt Analysis software 1.2 (Temse, Belgium). The percentage of confidence threshold was defined as 95%, below this threshold the result is considered as not acceptable as a true positive